Flow Cytometry Facility

The Flow Cytometry Facility is equipped with a user-friendly FACSCalibur flow cytometer available for use by investigators at Children's Memorial Research Center. The flow cytometer provides a convenient way to perform live cell immunophenotyping, viability study and DNA analysis.

Equipment

 FACSCalibur FACSCalibur: The FACSCalibur flow cytometer is equipped with a 488 nm argon laser and a 635 nm diode laser, which excite appropriate fluorochromes conjugated to antibodies used to stain cells. The cytometer can measure up to four fluorescent colors, as well as the scattering profile of cells (forward scattering and side scattering). Users can choose a combination of fluorochrome colors that best suit their experiments. Available fluorochromes include fluorescein (FITC) or Alexa Fluor 488 (green color, emission wavelength 520 nm), phycoerythrin (PE) (yellow color, 580 nm), PE-Cy5 or PE-Texas Red (red color, 670 nm), allophycocyanin (APC) or Alexa Fluor 647 (red color, 670 nm). Alternatively, expression of GFP, HcRed or DsRed in cells can be analyzed on the cytometer. DNA or RNA-binding dyes can be used in DNA analysis. The FACSCalibur runs the software program Cell Quest on a Power Macintosh, which can also be used for analysis of flow cytometry data. The charge for the use of the FACSCalibur is $30 per hour, billed to the cost center account of the investigator.
 FACSAria FACSAria: A FACSAria cell sorter is available in the High-Speed Cell Sorting Facility. The cell sorter is equipped with a 488 nm, 407 nm and 635 nm laser, which can measure up to 9 cellular markers and sterilely separate cells into 4 tubes at one time. Several common fluorochromes (FITC, PE, PE-Cy5, APC, PE-Cy7) and fluorescent proteins (GFP, DsRed, HcRed) have been used successfully. The use of the FACSAria is restricted.  More information.