Microarray Facility


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CHIDREN'S MEMORIAL RESEARCH CENTER

MICROARRAY FACILITY- HOME


 

CMRC Microarray Facility
Children’s Memorial Hospital in Chicago
Pediatric Brain Tumor Program Dr. Tadanori Tomita
2430 N. Halsted C.308
Chicago, IL 60614
Phone (773) 775-6364

Main Services:

Process samples starting from total RNA or cRNA.

FromTotal RNA

  • Synthesize double stranded cDNA from total RNA
  • Perfrom in vitro transcription reactions, with the double stranded cDNA as template, using a nuclotide analog/ ribonucleotide mix to amplify and label the cRNA respectively.
  • Fragmentation and hybridization of labeled cRNA on test chips. If samples pass QC, hybridization onto full length arrays
  • Washing and staining on fluidics 450 station
  • Scanning on the GC 3000 high resolution scanner
  • Basic analysis using MAS 5.1 to analize the image and generate an output data file and Spotfire to make scatterplots and do hierarchical clustering. (higher level analysis is available on a consulting or collaborative basis)

From cRNA

  • Hybridization of labeled cRNA on test chips. If samples pass QC, hybridization onto full length arrays
  • Washing and staining on fluidics 450 station
  • Scanning on the GC 3000 high resolution scanner
  • Basic analysis using MAS 5.1 to analize the image and generate an output data file and Spotfire to make scatterplots and do hierarchical clustering. (higher level analysis is available on a consulting or collaborative basis)

To submit samples for processing, please follow the following instructions:

  1. Make sure the sample concentration is sufficient prior to submission (1ug/ul for Total RNA and 20ug total for cRNA).
  2. Fill out a sample request form (see link on this website)
  3. Submit all RNA on dry ice.